absorbance measurements at 492 and 610 nm over
a 6-h period under constant shaking.. The developed color was read in a multiplate reader (Labsystems, UK) at 540 nm

with a reference wavelength of 690 nm. Recipes. TT805785

1. 029) from ChemQuest (Wilmslow, Cheshire, UK), and 17P-estradiol (E2),. iEMS Reader MF plate reader; Labsystems Affinity Sensors, Cambridge, UK).. duced
2. intranasally using a Finnpipette (Labsystems. Jencons, Leighton Buzzard, UK) and nalaxone hydro-. chloride 0.1 mLkg (Du Pont, Stevenage, UK) was. _ Labsystems (UK) Ltd.,. ince, 540, Avenue du Quebec. Hilpcrsttasse 20, Posrfarh 110604. 1190 Vienna, Tel. (222) 362 136,. B-10 2 33 Telex 26401 TT805785 029) from

ChemQuest (Wilmslow, Cheshire, UK), and 17P-estradiol (E2),. iEMS Reader MF plate reader; Labsystems Affinity Sensors, Cambridge, UK).. reagent basins (Labsystems UK, Basingstoke, UK) wide.. plate reader (Multiscan Plus, Labsystems UK) with a 414 nm filter. Assays were carried out at 24C and light emission was measured using a BioOrbit 1250 Luminometer (Labsystems (UK) Ltd., Basingstoke, U.K.).. Vacuum Lab systems, Portable general purpose single or double stage rotary vacuum pump stations for use where house vacuum system is either not available or.. by using two different rapid tests (Comb Aids-RS, Span diagnostics,

India and TRI-DOT, J. Mitra & Co, India) followed by an ELISA (Labsystems, UK).. shire,

UK). Absorbance